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Pyrosequencing Inc pyrosequencing analysis software
Pyrosequencing Analysis Software, supplied by Pyrosequencing Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pyrosequencing+analysis+software/pyrosequencing+software/pm35107180-102-49-49
Average 90 stars, based on 1 article reviews
pyrosequencing analysis software - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Software:

Article Title: Transcriptional activation of S100A2 expression by HIF-1α via binding to the hypomethylated hypoxia response elements in HCC cells.
Article Snippet: Funding information National Natural Science Foundation of China, Grant/Award Numbers: 81660024, 32060156; Natural Science Foundation of Inner Mongolia, Grant/Award Numbers: 2020MS08096, 2020MS03007 Abstract Hepatocellular carcinoma (HCC) is one of the most prevalent and lethal cancers.. Dysregulation of S100A2 has recently been found in many cancers including HCC.. However, its regulatory mechanism in HCC remains poorly understood, especially in hypoxia.

Article Title: Quantitative Analysis of Activating Alpha Subunit of the G Protein (Gs?) Mutation by Pyrosequencing in Fibrous Dysplasia and Other Bone Lesions
Article Snippet: .. Quantitative analysis of point mutation of the positions 1 and 2 of the Arg201 codon CGT was obtained by the pyrosequencing analysis software. ..

Mutagenesis:

Article Title: Quantitative Analysis of Activating Alpha Subunit of the G Protein (Gs?) Mutation by Pyrosequencing in Fibrous Dysplasia and Other Bone Lesions
Article Snippet: .. Quantitative analysis of point mutation of the positions 1 and 2 of the Arg201 codon CGT was obtained by the pyrosequencing analysis software. ..

Polymerase Chain Reaction:

Article Title: Αlpha 2a-Adrenoceptor Gene Expression and Early Life Stress-Mediated Propensity to Alcohol Drinking in Outbred Rats
Article Snippet: To verify the efficiency of sodium bisulfite DNA conversion, each individual Pyrosequencing reaction included a non-CpG cytosine as an internal bisulfite modification control, while low, medium, and high methylated DNA samples were included as controls in each plate. .. Also, pyrosequencing used to do PCR bias testing for samples made of a mix of the unmethylated DNA control and in vitro methylated DNA at different ratios (0%, 5%, 10%, 25%, 50%, 75% and 100%) followed by bisulfite modification, PCR and pyrosequencing analysis. ..

Control:

Article Title: Αlpha 2a-Adrenoceptor Gene Expression and Early Life Stress-Mediated Propensity to Alcohol Drinking in Outbred Rats
Article Snippet: To verify the efficiency of sodium bisulfite DNA conversion, each individual Pyrosequencing reaction included a non-CpG cytosine as an internal bisulfite modification control, while low, medium, and high methylated DNA samples were included as controls in each plate. .. Also, pyrosequencing used to do PCR bias testing for samples made of a mix of the unmethylated DNA control and in vitro methylated DNA at different ratios (0%, 5%, 10%, 25%, 50%, 75% and 100%) followed by bisulfite modification, PCR and pyrosequencing analysis. ..

In Vitro:

Article Title: Αlpha 2a-Adrenoceptor Gene Expression and Early Life Stress-Mediated Propensity to Alcohol Drinking in Outbred Rats
Article Snippet: To verify the efficiency of sodium bisulfite DNA conversion, each individual Pyrosequencing reaction included a non-CpG cytosine as an internal bisulfite modification control, while low, medium, and high methylated DNA samples were included as controls in each plate. .. Also, pyrosequencing used to do PCR bias testing for samples made of a mix of the unmethylated DNA control and in vitro methylated DNA at different ratios (0%, 5%, 10%, 25%, 50%, 75% and 100%) followed by bisulfite modification, PCR and pyrosequencing analysis. ..

Methylation:

Article Title: Αlpha 2a-Adrenoceptor Gene Expression and Early Life Stress-Mediated Propensity to Alcohol Drinking in Outbred Rats
Article Snippet: To verify the efficiency of sodium bisulfite DNA conversion, each individual Pyrosequencing reaction included a non-CpG cytosine as an internal bisulfite modification control, while low, medium, and high methylated DNA samples were included as controls in each plate. .. Also, pyrosequencing used to do PCR bias testing for samples made of a mix of the unmethylated DNA control and in vitro methylated DNA at different ratios (0%, 5%, 10%, 25%, 50%, 75% and 100%) followed by bisulfite modification, PCR and pyrosequencing analysis. ..

Modification:

Article Title: Αlpha 2a-Adrenoceptor Gene Expression and Early Life Stress-Mediated Propensity to Alcohol Drinking in Outbred Rats
Article Snippet: To verify the efficiency of sodium bisulfite DNA conversion, each individual Pyrosequencing reaction included a non-CpG cytosine as an internal bisulfite modification control, while low, medium, and high methylated DNA samples were included as controls in each plate. .. Also, pyrosequencing used to do PCR bias testing for samples made of a mix of the unmethylated DNA control and in vitro methylated DNA at different ratios (0%, 5%, 10%, 25%, 50%, 75% and 100%) followed by bisulfite modification, PCR and pyrosequencing analysis. ..



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Primer design

Journal:

Article Title: Basic Principles and Technologies for Deciphering the Genetic Map of Cancer

doi: 10.1007/s00268-008-9851-y

Figure Lengend Snippet: Primer design

Article Snippet: Open in a separate window Fig. 7 K-RAS mutations in codon 12 and 13 illustrated in Sanger chromatogram and 454 pyrosequencing Amplicon Variant Analysis software.

Techniques: Software, Sequencing, Amplification

Primer design. As one can see in the Design Template, there is a padding region of 300 base pairs (bp) before and after the exon where the forward and reverse primers can be designed. The amplicon includes the target region which in turn consists of the region of interest (exon and a padding of 15bp important in the splicing mechanism) and a region of 5-35 bp that is technical requirement of the 454 or Sanger respectively to give accurate sequencing results. Each primer (P1 and 2) contains a specific and a universal region (black and red regions). The specific region attaches to the gene of interest. This primer region is used during the amplification process (PCR) and becomes part of the amplicon. The universal primer used during the sequencing process hybridizes to the universal region allowing the sequencing of any amplicon.

Journal:

Article Title: Basic Principles and Technologies for Deciphering the Genetic Map of Cancer

doi: 10.1007/s00268-008-9851-y

Figure Lengend Snippet: Primer design. As one can see in the Design Template, there is a padding region of 300 base pairs (bp) before and after the exon where the forward and reverse primers can be designed. The amplicon includes the target region which in turn consists of the region of interest (exon and a padding of 15bp important in the splicing mechanism) and a region of 5-35 bp that is technical requirement of the 454 or Sanger respectively to give accurate sequencing results. Each primer (P1 and 2) contains a specific and a universal region (black and red regions). The specific region attaches to the gene of interest. This primer region is used during the amplification process (PCR) and becomes part of the amplicon. The universal primer used during the sequencing process hybridizes to the universal region allowing the sequencing of any amplicon.

Article Snippet: Open in a separate window Fig. 7 K-RAS mutations in codon 12 and 13 illustrated in Sanger chromatogram and 454 pyrosequencing Amplicon Variant Analysis software.

Techniques: Amplification, Sequencing

K-RAS mutations in codon 12 and 13 illustrated in Sanger chromatogram and 454 pyrosequencing Amplicon Variant Analysis software. The different intensity of the superposed bases in the Sanger chromatograms of the different patients illustrates the difficulty for an automatic call with SNP detector. On the other hand, in the 454 pyrosequencing, the mutations are successfully called by AVA. For this reason, the 454 pyrosequencing is a great discovery technology. However, the % given is not representative of the % of patients with the mutations because of factors such as heterogenous sample, heteroploidy and chromosomal loss. Genotyping with another technology then needs to be performed in each sample as well as in the matched normal tissue to determine which mutation is somatic and define the percentage of patients with each mutation.

Journal:

Article Title: Basic Principles and Technologies for Deciphering the Genetic Map of Cancer

doi: 10.1007/s00268-008-9851-y

Figure Lengend Snippet: K-RAS mutations in codon 12 and 13 illustrated in Sanger chromatogram and 454 pyrosequencing Amplicon Variant Analysis software. The different intensity of the superposed bases in the Sanger chromatograms of the different patients illustrates the difficulty for an automatic call with SNP detector. On the other hand, in the 454 pyrosequencing, the mutations are successfully called by AVA. For this reason, the 454 pyrosequencing is a great discovery technology. However, the % given is not representative of the % of patients with the mutations because of factors such as heterogenous sample, heteroploidy and chromosomal loss. Genotyping with another technology then needs to be performed in each sample as well as in the matched normal tissue to determine which mutation is somatic and define the percentage of patients with each mutation.

Article Snippet: Open in a separate window Fig. 7 K-RAS mutations in codon 12 and 13 illustrated in Sanger chromatogram and 454 pyrosequencing Amplicon Variant Analysis software.

Techniques: Amplification, Variant Assay, Software, Mutagenesis